An N-acetyl-d-glucosamine binding lectin from Bandeiraea Simplicifolia seeds
نویسندگان
چکیده
منابع مشابه
An -N-acetyl-D-glycosamine binding lectin from Bandeiraea simplicifolia seeds.
An cY-n-galactopyranosyl binding lectin (1) was recently isolated and characterized from the seed extracts ofBandeiraea simplicifolia. While pursuing further studies on this lectin, a second protein capable of interacting with p-azophenyl 2-acetamido-2deoxy-aand -p-n-glucopyranoside BSA’ conjugates was discovered. The new lectin, isolated from the same plant seed by affinity chromatography, sho...
متن کاملSerological studies on an alpha-D-galactosyl-binding lectin isolated from Bandeiraea simplicifolia seeds.
Abstruct. The serological characteristics of a highly purified a-D-galactosyl-binding lectin, isolated from extracts of Buntfeiraen siniplicifoliu seeds, are described. These studies show that the lectin preferentially agglutinates group B red cells and in addition has the capacity to distinguish between group A, and group A, erythrocytes. Since the lectin is not absolutely specific for group B...
متن کاملEquilibrium dialysis and cell binding studies on Bandeiraea simplicifolia lectin.
Equilibrium dialysis studies on the binding of the blood group B-specific Bandeiraea simplicifolia lectin to methyl alpha-D-galactopyranoside demonstrated the existence of one carbohydrate binding site per subunit for the tetrameric protein, with an intrinsic association constant of 8.6 x 10(4) (M) (-1) at 2 degrees and 3.3 x 10(4) (M)(-1) at 20 degrees. These values correspond to a free energy...
متن کاملImproved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography.
Bandeiraea simplicifolia plant seeds contain a family of five alpha-D-galactopyranosyl-binding isolectins (BS I-A4, A3B, A2B2, AB3, B4) and N-acetyl-D-glucosamine-binding lectin (BS II). After Pi/NaCl extraction and ammonium sulfate fractionation BS II is specifically adsorbed onto p-aminobenzyl-1-thio-N-acetyl-beta-D-glucosaminide-succinylaminohexylaminyl--Sepharose-4B. The BS I isolectins pas...
متن کاملBS II lectin: a second hemagglutinin isolated from Bandeiraea simplicifolia seeds with affinity for type III polyagglutinable red cells.
BS II lectin, a second hemagglutinin isolated from Bandeiraea simplicifolia seeds, agglutinated the red cells from 11 patients manifesting the 'acquired B' antigen phenomenon. The results of serological studies indicate that this lectin has specificity for the type III polyagglutinable receptors rather than for the 'B-like' antigens present on 'acquired B' red cells. Two other blood samples whi...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 1976
ISSN: 0003-9861
DOI: 10.1016/0003-9861(76)90444-6